Orbitrap LC/MS
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Thermo Scientific Finnigan LTQ
Orbitrap Elite with Velos Pro and ETD
Usage
At PPB Analytical Incorporated, we offer researchers to have the chance to use the laboratory equipment in our lab at an affordable price. Book a Orbitrap LC/MS capability with the Thermo Scientific Finnigan LTQ Orbitrap Elite with Velos Pro and ETD by calling us or sending an email.
Request A quote
To request a quote for the Orbitrap LC/MS capability using the Thermo Scientific Finnigan LTQ Orbitrap Elite with Velos Pro and ETD, click here.
Product Description
This system includes:
- (1) Thermo Scientific Velos Pro
- (1) Thermo Scientific Orbitrap Elite
- (1) Thermo Scentific Neslab ThermoFlex 900
- (1) Thermo Scientific ETD
- (2) Rough pump
- (1) PC with software
- (Optional) HPLC front end
Thermo Scientific Velos Pro
- Resolving power up to 100,000 FWHM and mass accuracy better than 1ppm RMS provide confidence when analyzing complex samples
- Multiple fragmentation techniques—CID, HCD, and optionally ETD—offer versatility and facilitate structural characterization
- Fast scanning and parallel MS and MSn analysis speeds analyses
- Ion optics with novel neutral-blocking technology increase robustness
- Collision cell with axial field gradient improves quantitative results
- High mass resolution and mass accuracy enable high-confidence molecular weight determination of intact proteins, peptide identification in complex samples and analysis of isobaric species
- High sensitivity increases detection of low-abundance peptides and proteins
- Short duty cycle increases sequence coverage within in a single experiment
- Stability, mass accuracy and wide dynamic facilitate label-free quantification
- Mass resolution and mass accuracy distinguish between heavy and light peptide ion pairs and interferences in SILAC experiments
- HCD cell with axial field improves quantification of isobarically labeled peptides
- Optional electron-transfer dissociation (ETD) generates complementary information for P™ analyses and de novo sequencing
Thermo Scientific Orbitrap Elite
Analyze demanding proteomics, metabolomics, lipidomics and metabolism samples in greater depth using the Thermo Scientific™ Orbitrap Elite™ Hybrid Ion Trap-Orbitrap Mass Spectrometer. This instrument combines a high-field Thermo Scientific™ Orbitrap™ mass analyzer and Thermo Scientific™ Velos Pro™ ion trap technology to deliver very high resolution, speed, sensitivity and complementary fragmentation information.
| Mass Range | 50 to 2000m/z; 200 to 4000m/z |
|---|---|
| Scan Rates | MSn for n = 1 Through 10 |
| Weight (Metric) | 620kg |
| Dynamic Range | >5000 within a single scan guaranteeing specified |
| Mass Accuracy | <3ppm RMS with external calibration; <1ppm RMS using internal calibration; <1ppm RMS using internal calibration |
| Resolution | 60,000 at m/z 400 at a scan rate of 4Hz, min. resolution 15,000, max. resolution >240,000 at m/z 400 |
| Voltage | 230V |
| Item Description | Orbitrap Elite hybrid ion trap-Orbitrap mass spectrometer |
| Description | Orbitrap Elite Hybrid Ion Trap-Orbitrap Mass Spectrometer |
| Unit Size | Each |
Thermo Scentific Neslab ThermoFlex 900
Optimized for the most demanding applications, Thermo Scientific™ ThermoFlex Recirculating Chillers are reliable, easy-to-use and easy-to-maintain. The ThermoFlex platform was developed with customer input from concept to design. This high-performance chiller platform, with cooling capacity up to 24,000 watts (81,948 btu/hr), is configurable to meet application demands.
| Configuration Options | Fluid Filtration (included), DI Support (Available), Auto Refill (Available), Flow Control with Readout (Available) |
|---|---|
| Description | ThermoFlex 900 Recirculating Chiller |
| Temperature Stability | &plusnm;0.1°C |
| Certifications/Compliance | CE, CSA |
| Communications | Analog & Digital (available) |
| Condenser Type | Air Cooled |
| Cooling Capacity | Up to 900 watts (60Hz) 750 watts (50Hz) |
| Pump Capacity | Up to 3.3 gpm @ 60 psig (12.5 L/min. @ 4.1 bar) (50Hz) Up to 4.0 gpm @ 60 psig (15. |
| Temperature Range | 5°C to 40°C |
| Unit Size | Each |
Thermo Scientific ETD
Achieve better analysis of proteins and their post-translational modifications (PTMs) with the Thermo Scientific™ Electron Transfer Dissociation (ETD) option. Unlike CID, which cleaves weakly bound modifications off the peptide backbone, ETD induces fragmentation along the peptide backbone in a sequence-independent manner, leaving labile modifications linked to the peptide chain. This greatly simplifies identification of modification sites. ETD produces primarily c- and z-type fragment ions that complement the b- and y-type ions produced by CID, increasing sequence coverage and protein IDs.
- ETD preserves labile PTMs, allowing straightforward identification of the peptide sequence and the site of modification
- For top-down analysis, ETD can be combined with proton transfer reaction (PTR) which dramatically simplifies spectra from intact proteins
- For workflows that include low-mass isobaric mass tags, ETD can be combined with pulsed-Q dissociation (PQD) which eliminates the normal low-mass cutoff
- ETD can be added to most Thermo Scientific LTQ-series ion trap and hybrid ion trap-Orbitrap mass spectrometers
- Proteome Discoverer software includes the proprietary Z-Core database search algorithm specifically optimized for ETD spectra
- Proteome Discoverer software can easily merge complementary CID and ETD results to maximize protein sequence coverage